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Authors:Cheon,S.A.;Han,E.J.;Kang,H.A.;Ogrydziak,D.M.;Kim,J.Y. 
Year:2003 
Journal:
 
Volume:20 
Keywords:GENBANK/AF420590 Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Fungal/chemistry/genetics/isolation & purification Fungal Proteins/*genetics/metabolism Genetic Complementation Test Genetic Markers/genetics Molecular Sequence Data Sequence Alignment Support, Non-U.S. Govt Yarrowia/enzymology/*genetics 
Abstract:The TRP1 gene encoding N-(5-phosphoribosyl)-anthranilate isomerase was isolated from the yeast Yarrowia lipolytica, in which only a few genetic marker genes are available. The Y. lipolytica TRP1 gene (YlTRP1) cloned by complementation of Y. lipolytica trp1 mutation was found to be a functional homologue of Saccharomyces cerevisiae TRP1. Since YlTRP1 could be used for counterselection in medium containing 5-fluoroanthranilic acid (5-FAA), we constructed TRP blasters that contained YlTRP1 flanked by a direct repeat of a sequence and allowed the recycling of the YlTRP1 marker. Using the TRP blasters the sequential disruption of target genes could be carried out within the same strain of Y. lipolytica. The nucleotide sequence of the YlTRP1 gene has been deposited at GenBank under Accession No. AF420590. 
Summary:Cheon,S.A.;Han,E.J.;Kang,H.A.;Ogrydziak,D.M.;Kim,J.Y. 2003. Yeast. 20 
Page:677-85 
Num_:8 
ISSN:0749-503X 
Revue:1519 
Url:12794929 
Document Reference #:10973