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General information

 
Taxonomy
 Taxon name:
 
 Name changes:Saccharomyces carlsbergensis; Saccharomyces pastorianus 
 Type of organism:Yeast 
 Status of the strain:Type of Saccharomyces carlsbergensis E.C. Hansen 
 Classification:
 
 
Collections
 Other collections:CCRC 21423;DBVPG 6033;IFO 1167;IGC 4457;JCM 7256;MUCL 38889;NCYC 396;NRRL Y-12693;CLIB 176;IFO 11023;PYCC 4457 
 Other strain numbers (personal numbers):Carlsberg bottom yeast 
 
Biosafety and restrictions
 Biological Safety Level:BSL-1 
 GMO:- 
 
Origin
 Category of substrate:brewer 
 Country:
 
 Identified by:A. Vaughn-Martini & Martini, 1997 
 Deposited by:E.J. Helm, Carlsberg bottom yeast no.1 
 Substrate (including host):brewers yeast 
 
Miscellaneous information
 Miscellaneous information:[Applications]: brewers bottom yeast 
 Price per culture:170 

Media and growth conditions

 Medium for growth:
 
 Temperature, pH, Salinity & Ethanol information:[Salinity max]: 10- W/V
[Ethanol max]: <10 V/V
 

Sexuality

 Conditions for sporulation:McClary acetate agar, 12d, 25C 

Physiology

 
Fermentation
 D-Glucose (F1):positive 
 D-Galactose (F2):positive 
 Maltose (F3):positive 
 Me a-D-Glucoside (F4):weak, delayed 
 Sucrose (F5):positive 
 a,a-Trehalose (F6):negative 
 Melibiose (F7):positive 
 Lactose (F8):negative 
 Cellobiose (F9):negative 
 Melezitose (F10):negative 
 Raffinose (F11):positive 
 Inulin (F12):negative 
 Starch (F13):negative 
 D-Xylose (F14):negative 
 
Assimilation-Growth
  50% D-Glucose (O4):no growth 
 Starch formation (M1):no 
 Acid acetic production (M2):no 
 Urea hydrolysis (M3):no 
 Diazonium Blue B reaction (M4):negative 
 Methanol (C41):no growth 
 Ethanol (C42):growth 
 
Yeasts physiological data:
C1 D-Glucose +
C2 D-Galactose +
C3 L-Sorbose -
C4 D-Glucosamine -
C5 D-Ribose -
C6 D-Xylose -
C7 L-Arabinose -
C8 D-Arabinose -
C9 L-Rhamnose -
C10 Sucrose +
C11 Maltose +
C12 a,a-Trehalose d
C13 Me a-D-Glucoside d
C14 Cellobiose -
C15 Salicin -
C16 Arbutin -
C17 Melibiose +, d, w
C18 Lactose -
C19 Raffinose +
C20 Melezitose -
C21 Inulin -
C22 Starch -
C23 Glycerol +
C24 Erythritol -
C25 Ribitol -
C26 Xylitol -
C27 L-Arabinitol -
C28 D-Glucitol -
C29 D-Mannitol -
C30 Galactitol -
C31 myo-Inositol -
C32 D-Glucono-1,5-lactone -
C33 2-Keto-D-Gluconate -
C34 5-Keto-D-Gluconate -
C35 D-Gluconate -
C36 D-Glucuronate -
C37 D-Galacturonate -
C38 DL-Lactate +
C39 Succinate -
C40 Citrate -
C43 Propane 1,2 diol -
C44 Butane 2,3 diol -
C45 Quinic acid -
C46 D-glucarate -
C47 D-Galactonate -
C48 Palatinose ?
C49 Levulinate ?
C50 L-Malic acid ?
 
C51 L-Tartaric acid ?
C52 D-Tartaric acid ?
C53 meso-Tartaric acid ?
C54 Galactaric acid ?
C55 Uric acid ?
C56 Gentobiose ?
C57 Ethylene glycol ?
C58 Tween 40 ?
C59 Tween 60 ?
C60 Tween 80 ?
N1 Nitrate -
N2 Nitrite -
N3 Ethylamine -
N4 L-Lysine -
N5 Cadaverine -
N6 Creatine -
N7 Creatinine -
N8 Glucosamine -
N9 Imidazole -
N10 D-Tryptophan -
N11 D-Proline ?
N12 Putrescine ?
V1 w/o vitamins -
V2 w/o myo-Inositol +
V3 w/o Pantothenate +
V4 w/o Biotin -
V5 w/o Thiamin +
V6 w/o Biotin & Thiamin -
V7 w/o Pyridoxine +
V8 w/o Pyridoxine & Thiamin +
V9 w/o Niacin +
V10 w/o PABA +
O1 Cycloheximide 0.01% -
O2 Cycloheximide 0.1% ?
O3 Acetic acid 1% -
O6 10% NaCl ?
O7 16% NaCl ?
O8 Growth at pH=3 ?
O9 Growth at pH=9.5 ?
O10 Fluconazole ?
? ?
? ?
? ?
? ?
? ?
? ?
? ?
? ?
 

Bibliography

 Other bibliographic data:Lit: Hansen, CR.Trav.Lab.Carlsberg 7: 179-217, 1908; Rodrigues de Sousa et al., System.Appl.Microbiol. 18: 44-51, 1995 (fructose symport)
GenLit: Vaughan Martini & Kurtzman, Int.J.Syst.Bacteriol. 35: 508-511, 1985 (DNA); Pedersen, Carlsberg Res.Commun. 51: 163-183, 185-202, 1986 (karyotype); Piskur et al., Int.J.System.Bacteriol. 48: 1015-1024, 1998 (mitochondria
EcoLit:
PropLit:
PathLit:
ChLit:
AppLit:
 

DNA

 
Other information
 Plasmids:RNA, 5kbp <> H. Fukuhara 
 
Sequences
 rDNA sequences 26S:
 
 rDNA sequences 18S:
 
 rDNA sequences ITS:
 
 Elongation Factor 1: